Chemical Exposure-Related Metabolism Alterations in Mammalian CellCultures

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Metabolomic Approaches to Examine Chemical Publicity-Associated Metabolism Alterations in Mammalian CellCultures

Natural organisms are constantly uncovered to an immense repertoire of molecules that cowl environmental or food-derived molecules and medicines, triggering a gradual motion of stimuli-dependent variations.

 

  • The number of these chemical substances along with their concentrations contribute to the multiplicity of induced outcomes, along with activation, stimulation, or inhibition of physiological processes and toxicity. Metabolism, as a result of the foremost phenotype and manifestation of life, has confirmed to be immensely delicate and intensely adaptive to chemical stimuli.

 

  • Subsequently, studying the impression of endo- or xenobiotics over cellular metabolism delivers priceless info to apprehend potential cellular train of specific particular person molecules and contemplate their acute or energy benefits and toxicity.

 

  • The occasion of latest metabolomics utilized sciences akin to mass spectrometry or nuclear magnetic resonance spectroscopy now provides unprecedented choices for the speedy and surroundings pleasant willpower of metabolic profiles of cells and additional difficult natural strategies. Blended with the availability of well-established cell custom methods, these analytical methods appear fully suited to seek out out the natural train and estimate the constructive and antagonistic outcomes of chemical substances in numerous cell kinds and fashions, even at hardly detectable concentrations.

 

  • Metabolic phenotypes shall be estimated from studying intracellular metabolites at homeostasis in vivo, whereas in vitro cell cultures current further entry to metabolites exchanged with progress media.

 

  • This textual content discusses analytical choices on the market for metabolic phenotyping of cell custom metabolism along with the general metabolomics workflow acceptable for testing the natural train of molecular compounds.

 

  • We emphasize how metabolic profiling of cell supernatants and intracellular extracts can ship priceless and complementary insights for evaluating the results of xenobiotics on cellular metabolism. We phrase that the concepts and methods talked about primarily for xenobiotics publicity are extensively related to drug testing on the entire, along with endobiotics that cowl energetic metabolites, nutritional vitamins, peptides and proteins, cytokines, hormones, dietary nutritional vitamins, and so forth.

 

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FluoreGuard Mounting Medium
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FluoreGuard Mounting Medium
FMM999 1000 ml
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EUR 201
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23001 10mL
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Fluorguard Antifade Mounting Medium
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EUR 86
Anti-Fade Mounting Medium
abx090692-10ml 10 ml
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EverBrite Hardset Mounting Medium
23003 10mL
EUR 195
Description: Minimum order quantity: 1 unit of 10mL
Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 60ml
NB300 60 ml
EUR 427
Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 30ml
NB300-30 30 ml
EUR 276
Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 125ml
NB300A 125 ml
EUR 543
FluoreGuard Mounting Medium (Hard Set)
FMH030 30 ml
EUR 96
FluoreGuard Mounting Medium (Hard Set)
FMH060 60 ml
EUR 116
FluoreGuard Mounting Medium (Hard Set)
FMH500 500 ml
EUR 327
FluoreGuard Mounting Medium (Hard Set)
FMH999 1000 ml
EUR 530
EverBrite Mounting Medium with DAPI
23002 10mL
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Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml 10 ml
EUR 203
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Anti-Fade Mounting Medium (With PI)
abx090691-10ml 10 ml
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EverBrite Hardset Mounting Medium with DAPI
23004 10mL
EUR 213
Description: Minimum order quantity: 1 unit of 10mL
FluoroQuest™ Mounting Medium with DAPI
20004 50 mL
EUR 132
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Mounting Media
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EUR 211
Description: Mounting media for use in microscopy preparations
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EUR 142
Buffered Glycerol Mounting Media
GMM003 3 ml
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Buffered Glycerol Mounting Media
GMM030 30 ml
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Buffered Glycerol Mounting Media
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Buffered Glycerol Mounting Media
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EUR 260
Permanent Mounting Media (Aqueous)
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Permanent Mounting Media (Aqueous)
PMT999 1000 ml
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ReadiUse™ microscope mounting solution
20009 50 mL
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Fine natural hair brush for mounting sections
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Medium 199
C0012-01 RT 500 mL Bottle
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COLIFORM MEDIUM
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EC MEDIUM
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EC MEDIUM
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EC MEDIUM
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HLP MEDIUM
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HLP MEDIUM
H08-107-2Kg 2 Kg
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HLP MEDIUM
H08-107-500g 500 g
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450 500 ml
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BEGM Medium
451 500 ml
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A Medium
DJ1018 100g
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DC MEDIUM
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DC MEDIUM
D04-117-2kg 2kg
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DC MEDIUM
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EUR 140
Heller's Medium
CP014-010 10X1L
EUR 99
Heller's Medium
CP014-500 50L
EUR 126
Hoagland's Medium
CP015-010 10X1L
EUR 99
Hoagland's Medium
CP015-500 50L
EUR 126
NeuroProgenitor Medium
NM42400 125 ml
EUR 304
SIM MEDIUM
S19-110-10kg 10 kg
EUR 1021
SIM MEDIUM
S19-110-2kg 2kg
EUR 261
SIM MEDIUM
S19-110-500g 500 g
EUR 107
SOB MEDIUM
S19-124-10kg 10 kg
EUR 965
SOB MEDIUM
S19-124-2kg 2kg
EUR 249
SOB MEDIUM
S19-124-500g 500 g
EUR 104
M9 Medium
SD7024 250g
EUR 71.75
  • Product category: Culture Media/Medium
M9CA Medium
SD7025 250g
EUR 71.75
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YM Medium
SD7031 250g
EUR 70.45
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TYGPN Medium
SD7032 250g
EUR 70.01
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M63 Medium
SD7033 250g
EUR 71.75
  • Product category: Culture Media/Medium
Probe Mount, Permanent Mounting media for permanent mounting and coverslipping of Special stainsd & Immunofluorescence and In-Situ Stains 60ml
NB316 60 ml
EUR 485
Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001 1 liter Ask for price
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ABP-MED-10002 1 liter Ask for price
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ABP-MED-10004 1 liter Ask for price
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C0002-20mL RT vial
EUR 104
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C03-131-2Kg 2 Kg
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EC MEDIUM, MODIFIED
E05-108-10kg 10 kg
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E05-108-2Kg 2 Kg
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EC MEDIUM, MODIFIED
E05-108-500g 500 g
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HM42600 100 ml
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HNM001 500 ml
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GE4750-500G 500 g
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F06-101-10kg 10 kg
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F06-101-2kg 2kg
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F06-101-500g 500 g
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RPMI 1640 Medium
abx098879-500ml 500 ml
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A01-116-10kg 10 kg
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A-1 MEDIUM
A01-116-2Kg 2 Kg
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1711 4/PK
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DJ1019 100g
EUR 60.88
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MCDB 131 Medium
CM034-050 500ml
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MCDB 131 Medium
CM034-300 6x500ml
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MCDB 131 Medium
CM034-310 10x500ml
EUR 219
MCDB 131 Medium
CM034-320 20x500ml
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MCDB 131 Medium
CM034-350 50x500ml
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Williams' Medium E
CM063-050 500ml
EUR 91
Williams' Medium E
CM063-300 6x500ml
EUR 266

 

Conditional reprogramming: subsequent know-how cellculture

 

 

Prolonged-term main custom of mammalian cells has been always powerful attributable to unavoidable senescence. Commonplace methods for producing immortalized cell strains usually require manipulation of genome which results in change of significant natural and genetic traits. Not too way back, conditional reprogramming (CR) emerges as a novel subsequent know-how instrument for long-term custom of main epithelium cells derived from practically all origins with out alteration of genetic background of main cells.

 

CR co-cultures main cells with inactivated mouse 3T3-J2 fibroblasts throughout the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling main cells to build up stem-like traits whereas retain their potential to fully differentiate. With just some years’ progress, CR reveals broad prospects in functions in diversified areas along with sickness modeling, regenerative medication, drug evaluation, drug discovery along with precision medication. This consider is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its broad functions, highlighting the value of CR in every major and translational researches and discussing the challenges confronted with CR.

 

Outcomes of Kifunensine on Manufacturing and N-Glycosylation Modification of Butyrylcholinesterase in a Transgenic Rice CellCulture Bioreactor

 

The manufacturing and N-glycosylation of recombinant human butyrylcholinesterase (BChE), a model extraordinarily glycosylated therapeutic protein, in a transgenic rice cell suspension custom dealt with with kifunensine, a robust α-mannosidase I inhibitor, was studied in a 5 L bioreactor.

 

A media commerce was carried out at day 7 of cultivation by eradicating spent sugar-rich medium (NB+S) and together with current sugar-free (NB-S) medium to induce the rice α-amylase 3D (RAmy3D) promoter to offer rice recombinant human BChE (rrBChE). Using a 1.25X-concentrated sugar-free medium together with an 80% lowered working amount by way of the media commerce led to a whole energetic rrBChE manufacturing diploma of 79 ± 2 µg (g FW)-1 or 7.5 ± 0.4 mg L-1 throughout the presence of kifunensine, which was 1.5-times better than our earlier bioreactor runs using common sugar-free (NB-S) media with no kifunensine treatment.

 

Importantly, the amount of secreted energetic rrBChE in custom medium was enhanced throughout the presence of kifunensine, comprising 44% of your complete energetic rrBChE at day 5 following induction. Coomassie-stained SDS-PAGE gel and Western blot analyses revealed fully completely different electrophoretic migration of purified rrBChE bands with and with out kifunensine treatment, which was attributed to fully completely different N-glycoforms. N-Glycosylation analysis confirmed significantly elevated oligomannose glycans (Man5/6/7/8) in rrBChE dealt with with kifunensine compared with controls. Nonetheless, the mass-transfer limitation of kifunensine was seemingly the principle function for incomplete inhibition of α-mannosidase I on this bioreactor look at.

 

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SODIUM THIOGLYCOLLATE

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A01-103-2Kg 2 Kg Ask for price

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A01-103-500g 500 g Ask for price

Bouin's Fluid

BNF125 125 ml
EUR 63

Bouin's Fluid

BNF3800 1 Gal.
EUR 178

Bouin's Fluid

BNF500 500 ml
EUR 80

Bouin's Fluid

BNF999 1000 ml
EUR 98

Rat Neuromedin U control/blocking peptide # 2

NMU51-P 100 ug
EUR 164

Human Neuromedin U control/blocking peptide # 3

NMU61-P 100 ug
EUR 164

Mouse Neuromedin U control/blocking peptide # 4

NMU71-P 100 ug
EUR 164

P-Selectin (PF377), RNA Aptamer, unlabeled

AR-298-U Custom Ask for price

Michel's Transport Fluid

MTF-10000 10 L
EUR 762

Michel's Transport Fluid

MTF-20000 20 L
EUR 1340

Michel's Transport Fluid

MTF500 500 ml
EUR 98

Michel's Transport Fluid

MTF999 1000 ml
EUR 127

Ansamitocin P-3

ADC-P-003 unit Ask for price

Neuromedin U (R/M/H) control/blocking peptides

NMU41-P 100 ug
EUR 164

SOF Synthetic Oviduct Fluid.

IVL05-100ML 100 ml
EUR 78
  • Product line: Animal Cell Culture Media
  • Product family: Bovine IVF Media
Description: Synthetic Oviduct Fluid.

SOF Synthetic Oviduct Fluid.

IVL05-6X100ML 6 x 100 ml
EUR 151
  • Product line: Animal Cell Culture Media
  • Product family: Bovine IVF Media
Description: Synthetic Oviduct Fluid.

HE4, Human ascites fluid

P1438-100
EUR 620

HE4, Human ascites fluid

P1438-500
EUR 2839

AXYPREP MAG PLASMID KIT- MEDIUM - 384 PREPS

MAG-P-M 1/pk
EUR 664
Description: Bioscience Mag Beads; Magnetic Plasmid

Transparent, 96-Well Plates, pureGrade Sterile, Clear U-Bottom 50 Plates/Unit

91-420U 50 plates
EUR 230
Description:
  • Non-treated medium binding surface
  • 100% high quality polystyrene
  • Individually-wrapped plates w/ lids
  • Sterilized via beta-radiation
  • Available in clear, white or black
  • Free from endotoxins, DNase, DNA, RNase; noncytotoxic

96-Well, pureGrade, Clear Transparent, U-Bottom, 5 Plates/Sleeve, 100/Unit

33-572X 100 plates
EUR 240
Description:
  • Clear for colorimetric assays
  • Non-treated "medium binding" surface
  • 100% high quality polystyrene
  • 330µl well volume
  • Non-sterile
  • 1 lid included in each stack (20 total lids)
  • 20 stacks of 5 plates, pack of 100

YLKKIKNSL, P. falciparum circumsporozoite (CSP 334-342) polypeptide

CSPF15-P 1 mg
EUR 286

Mouse p/CIP/p300 Control/blocking peptide # 1

CIP11-P 100 ug
EUR 164

Mouse p/CIP/p300 Control/blocking peptide # 2

CIP12-P 100 ug
EUR 164

Hepatitis B Virus (HBV) Polymerase (P protein) (A9), RNA Aptamer, unlabeled

AR-233-U Custom Ask for price

Calci-Clear

NAT1312 EACH
EUR 78

Calci-Clear

NAT1314 EACH
EUR 128

Calci-Clear

NAT1316 EACH
EUR 386

Histo-Clear

NAT1330 EACH
EUR 154

Histo-Clear

NAT1332 EACH
EUR 583

LEYYLREKAKMAGTLIIPES, P. vivax PvMSP-1 peptide 19 (378-397)

MSPV11-P 1 mg
EUR 164

SKDQIKKLTSLKNKLERRQN, P. vivax PvMSP-1 peptide 53 (1058-1077)

MSPV12-P 1 mg
EUR 164

NFVGKFLELQIPGHTDLLHL, P. vivax PvMSP-1 peptide 4 (78-97)

MSPV13-P 1 mg
EUR 164

FNQLMHVINFHYDLLRANVH, P. vivax PvMSP-1 peptide 6 (118-137)

MSPV14-P 1 mg
EUR 164

LDMLKKVVLGLWKPLDNIKD, P. vivax PvMSP-1 peptide 8 (158-177)

MSPV15-P 1 mg
EUR 164

Human Substance P Peptide (amide salt)

SP15-P-1 1 mg
EUR 164

Human Substance P Peptide (amide salt)

SP15-P-25 25 mg
EUR 529

Human Substance P Peptide (amide salt)

SP15-P-5 5 mg
EUR 286

CA-125, Human Ascites Fluid

P1446-10
EUR 294

MICROPLATE, 96 WELL, POLYSTYRENE, CLEAR, FLAT BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, BULK

9017 25/pk
EUR 179
Description: Assay; General Assay - 96 Well

MICROPLATE, 96 WELL, POLYSTYRENE, CLEAR, ROUND BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, BULK

3797 25/pk
EUR 179
Description: Assay; EIA/RIA - 96 Well

KIYNRNIVNRLLGD, P-yoelii circumsporozoite protein (57?70), PyCSP (57?70) peptide

CSPY11-P 1 mg
EUR 286

SYVPSAEQI, P-yoelii circumsporozoite protein (280?288), PyCSP (280?288) peptide

CSPY12-P 1 mg
EUR 286

DELFNELLNSVDVNGENILEESQ, P. falciparum Liver-Stage Antigen 3-NRI (LSA3-NRI) peptide

LSPF32-P 1 mg
EUR 286

U-44069

C5324-1 1 mg
EUR 224
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U-44069

C5324-10 10 mg
EUR 1442
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U-44069

C5324-5 5 mg
EUR 833
Description: EC50: 3 ?M and 54 nM for platelet aggregation and phosphatidate formation in human platelets, respectivelyU-44069 is a TP receptor agonist.

U-93631

B1851-25
EUR 544

U-93631

B1851-5
EUR 175

U-73122

B1887-25
EUR 544

U-73122

B1887-5
EUR 175

U-73343

B1888-5
EUR 175

U-73122

B3422-10 10 mg
EUR 161
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-25 25 mg
EUR 258
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-5 5 mg
EUR 123
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U-73122

B3422-5.1 10 mM (in 1mL DMSO)
EUR 166
Description: U-73122 is an inhibitor of phospholipase C, phospholipase A2, and 5-LO (5-lipoxygenase).

U 18666A

B6812-10 10 mg
EUR 150
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 18666A

B6812-25 25 mg
EUR 287
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 18666A

B6812-50 50 mg
EUR 473
Description: U 18666A is an inhibitor of cholesterol transport and synthesis [1] [2]. Cholesterol is a sterol that biosynthesized by all animal cells and is an essential component of all animal cell membranes that is required to maintain membrane fluidity and structural integrity.

U 46619

B6890-1 1 mg
EUR 147
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

U 46619

B6890-10 10 mg
EUR 830
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

U 46619

B6890-5 5 mg
EUR 487
Description: EC50: 0.035 ?M for shape change, 0.057 ?M for MLCP, 0.536 ?M for serotonin release, 1.31 ?M for aggregation.

U 90042

B7164-10 10 mg
EUR 350

U 90042

B7164-50 50 mg
EUR 1266

U 89843A

B7165-10 10 mg
EUR 292

U 89843A

B7165-50 50 mg
EUR 1054

U 93631

B7170-10 10 mg
EUR 248

U 93631

B7170-100 100 mg
EUR 1407

U 93631

B7170-5 5 mg
EUR 195

U 93631

B7170-5.1 10 mM (in 1mL DMSO)
EUR 209

U 93631

B7170-50 50 mg
EUR 864

U 73343

B9013-10 10 mg
EUR 224

U 73343

B9013-25 25 mg
EUR 441

U 73343

B9013-5 5 mg
EUR 154

U 0126

E1B1901 1mg
EUR 161

U-73343

HY-108630 5mg
EUR 234

U-101017

HY-19250 1mg
EUR 1125

U-73122

HY-13419 10mM/1mL
EUR 147

U-104

HY-13513 10mM/1mL
EUR 113

U-74389G

20-abx076631
  • EUR 314.00
  • EUR 857.00
  • 100 mg
  • 500 mg
  • Shipped within 5-12 working days.

U-0126

20-abx076796
  • EUR 1121.00
  • EUR 384.00
  • 25 mg
  • 5 mg
  • Shipped within 5-12 working days.

U-18666A

9469-100 Ask for price

U-18666A

9469-25
EUR 457

U-18666A

9469-5
EUR 153

U-104

9631-25
EUR 414

U-104

9631-5
EUR 142

U-104

A4358-10 10 mg
EUR 108
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-25 25 mg
EUR 171
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-5.1 10 mM (in 1mL DMSO)
EUR 108
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

U-104

A4358-50 50 mg
EUR 270
Description: U-104 is a novel ureido-sulfonamide inhibitor of Carbonic Anhydrase IX (CAIX). Tumor volume measurements show important inhibition of primary tumor growth in the mice treated with the U-104 compound compared with vehicle controls

Kazinol U

TBW00590 unit Ask for price

Gomisin U

TBW01615 5mg Ask for price

Ixerin U

TBZ1502 unit Ask for price

MICROPLATE, 96 WELL, POLYSTYRENE, CLEAR, FLAT BOTTOM, MEDIUM BINDING, NO LID, NONSTERILE, INDIVIDUALLY PACKAGED

3591 1/pk
EUR 130
Description: Assay; EIA/RIA - 96 Well

EZ-10 RNA Column and collection tube (clear tube, clear ring, clear collection)

SD5008 100, 100UNIT
EUR 75.88
  • Product category: Labware/Columns/Spin Columns

VTHESYQELVKKLEALEDAV, MSP-1 P1, peptide of P. falciparum Merozoite Surface Protein 1

MSPF11-P 1 mg
EUR 225

GYRKPLDNIKDNVGKMEDYIKK, MSP-1 P2, peptide of P. falciparum Merozoite Surface Protein 1

MSPF12-P 1 mg
EUR 225

KLNSLNNPHNVLQNFSVFFNK, MSP-1 P3, peptide of P. falciparum Merozoite Surface Protein 1

MSPF131-P 1 mg
EUR 225

384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR

PCR-384-RGD-C 20/pk
EUR 459
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Exosome Isolation kit (forHuman Body Fluid)

P121 NULL
EUR 0

Exosome Isolation kit (forHuman Body Fluid)

P121L NULL
EUR 0

Exosome Isolation kit (forHuman Body Fluid)

P121S NULL
EUR 0

Cancer Antigen 125 Antigen (Ascites Fluid)

VAng-Cr3870-10kU 10 kU
EUR 944
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE.

Calci-Clear Rapid

NAT1318 EACH
EUR 75

Calci-Clear Rapid

NAT1320 EACH
EUR 128

Calci-Clear Rapid

NAT1322 EACH
EUR 386

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