Variations in drug sensitivity between two-dimensional and three-dimensional

Zachary October 5, 2020 0 Comments
wildpalm

Variations in drug sensitivity between two-dimensional and three-dimensional custom strategies in triple-negative breast most cancers cell strains

 

Three-dimensional (3D) custom shows tumor biology complexities in distinction with two-dimensional (2D) custom. Thus, 3D custom has attracted consideration in cell biology analysis along with drug sensitivity exams.

 

Herein, we investigated variations in anticancer drug sensitivities between 2D and 3D custom strategies in triple-negative breast most cancers (TNBC) cell strains. 13 TNBC cell strains have been maintained in 2D and 3D cultures for Three days sooner than drug publicity. Cell morphology inside the 3D custom was examined by phase-contrast microscopy.

 

Sensitivities to epirubicin (EPI), cisplatin (CDDP), and docetaxel (DTX) have been investigated by cell viability assay in every cultures and in distinction. The IC50s of all Three treatment have been significantly better inside the 3D custom than inside the 2D custom in most cell strains.

 

These have been correlated between the 2D and 3D cultures in EPI (R = 0.555) and CDDP (R = 0.955), nonetheless not in DTX (R = 0.221). Spherical spheroid-forming cells have been further proof towards brokers than grape-like types. In conclusion, 3D custom was further proof towards all Three treatment than 2D custom in most TNBC cell strains. Sensitivity to CDDP was extraordinarily correlated between the 2D and 3D cultures, nonetheless to not DTX. 2D custom is also acceptable for sensitivity check out for DNA-damaging brokers.

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DC MEDIUM W/ BCIG
D04-116-10kg 10 kg
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D04-116-2Kg 2 Kg
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DC MEDIUM W/ BCIG
D04-116-500g 500 g
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BME 100X Vitamins for Basal Medium Eagle (Modified)
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  • Product line: Animal Cell Culture Media
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BME 100X Vitamins for Basal Medium Eagle (Modified)
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Gamborg's B-5 Medium; With Vitamins and Sucrose
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Gamborg's B-5 Medium; With Vitamins and Sucrose
CP012-500 50L
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Schneider's Medium, w/ L-glutamine
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Medium 199, with L-Glutamine, w/ Earle's saltsĀ 
CCM2441-500 500 mL
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Murashige and Skoog, With Vitamins
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Murashige and Skoog, With Vitamins
CP030-500 50L
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Glutamate dehydrogenase (40 U/mg), Beef Liver, freeze dried powder
GDH-B5 20 mU
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100 ML MEM VITAMINS 100X SOLUTION
25-020-CI 100 mL/pk
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Murashige and Skoog, With Gamborg's Vitamins
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Murashige and Skoog, With Gamborg's Vitamins
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Coxsackievirus (B5)
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Procyanidin B5
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Murashige and Skoog, With Vitamins and Glycine
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Rat leukotriene B5(LT-B5) ELISA kit
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  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat leukotriene B5 (LT-B5) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat leukotriene B5(LT-B5) ELISA kit
1-CSB-EQ027966RA
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  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat leukotriene B5(LT-B5) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

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Gluten Exorphin B5
H-1666.0025 25.0mg
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Description: Sum Formula: C30H38N6O7; CAS# [68382-18-3]

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Serpin B5 antibody
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Description: Rabbit polyclonal Serpin B5 antibody

Serpin B5 Antibody
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Serpin B5 Antibody
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Gluten Exorphin B5
5-01227 4 x 5mg Ask for price

Coxsackievirus B5 protein
30-1334 100 ug
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Description: Purified native Coxsackievirus B5 protein (Faulkener Strain)

Cytochrome B5 antibody
20R-CG009 100 ul
EUR 457
Description: Goat polyclonal Cytochrome B5 antibody

Vitamin B5 [HRP]
DAGA-123H 1mg
EUR 1222

Vitamin B5 [KLH]
DAGA-123K 1mg
EUR 1235

anti-Cytochrome b5
YF-PA11223 50 ul
EUR 363
Description: Mouse polyclonal to Cytochrome b5

anti-Cytochrome b5
YF-PA23551 50 ul
EUR 334
Description: Mouse polyclonal to Cytochrome b5

W 54011
B6069-10 10 mg
EUR 393
Description: W 54011 is a potent and orally active non-peptide C5a receptor antagonist with Ki value of 2.2 nM [1].The complement C5a is a 74-amino acid peptide produced during complement activation processes.

W 54011
B6069-25 25 mg
EUR 857
Description: W 54011 is a potent and orally active non-peptide C5a receptor antagonist with Ki value of 2.2 nM [1].The complement C5a is a 74-amino acid peptide produced during complement activation processes.

W 54011
B6069-5 5 mg
EUR 232
Description: W 54011 is a potent and orally active non-peptide C5a receptor antagonist with Ki value of 2.2 nM [1].The complement C5a is a 74-amino acid peptide produced during complement activation processes.

W-2429
HY-100174 1mg
EUR 567

W-54011
HY-16992A 10mM/1mL
EUR 288

Bcl-w
GT15196 100 ug
EUR 526

Compound W
A4401-50 50 mg
EUR 177
Description: Inhibitor of ?-secretase; causes a decrease in the released levels of A?42 and notch-1 A?-like peptide 25 (N?25).

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5-02089 4 x 5mg Ask for price

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DJ1018 100g
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Monoclonal Proinsulin Antibody (Clone HPI-B5), Clone: HPI-B5
AMR09558G 0.1mg
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Description: A Monoclonal antibody against Human Proinsulin (Clone HPI-B5). The antibodies are raised in Mouse and are from clone HPI-B5. This antibody is applicable in WB and IHC, E

Phytic acid, 50% (w/w) in water
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Beryllium fluoride, 33% (w/w) aqueous solution
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Rat Neuropeptide W(NP-W)ELISA Kit
CSB-E17025r-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
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Description: Quantitativesandwich ELISA kit for measuring Rat Neuropeptide W (NP-W) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Neuropeptide W(NP-W)ELISA Kit
1-CSB-E17025r
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  • 1 plate of 96 wells
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  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Neuropeptide W(NP-W) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Monoclonal GLP-1 Antibody (Clone HGL-B5), Clone: HGL-B5
AMM04792G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human GLP-1 (Clone HGL-B5). The antibodies are raised in Mouse and are from clone HGL-B5. This antibody is applicable in IF, E

Polyclonal anti-Cytochrome b5
Cb5 50 uL
EUR 280

Polyclonal Serpin B5 Antibody
APR05559G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Serpin B5 . This antibody is tested and proven to work in the following applications:

Serpin B5 Conjugated Antibody
C34298 100ul
EUR 397

anti- Cytochrome b5 antibody
FNab02186 100Āµg
EUR 505.25
  • Immunogen: cytochrome b5 type A(microsomal)
  • Uniprot ID: P00387
  • Research Area: Metabolism
Description: Antibody raised against Cytochrome b5

Cytochrome b5 Polyclonal Antibody
ES2121-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Cytochrome b5 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Cytochrome b5 Polyclonal Antibody
ES2121-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Cytochrome b5 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Serpin B5 (SERPINB5) Antibody
20-abx127043
  • EUR 411.00
  • EUR 592.00
  • 100 ul
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Serpin B5 (SERPINB5) Antibody
20-abx115469
  • EUR 732.00
  • EUR 398.00
  • 150 ul
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Serpin B5 (SERPINB5) Antibody
20-abx001093
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
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Serpin B5 Blocking Peptide
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  • EUR 272.00
  • EUR 411.00
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Serpin B5 (SERPINB5) Antibody
abx038026-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Cytochrome b5 Polyclonal Antibody
ABP51122-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of Cytochrome b5 from Human, Mouse, Rat. This Cytochrome b5 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110

Cytochrome b5 Polyclonal Antibody
ABP51122-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of Cytochrome b5 from Human, Mouse, Rat. This Cytochrome b5 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110

Cytochrome b5 Polyclonal Antibody
ABP51122-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of Cytochrome b5 from Human, Mouse, Rat. This Cytochrome b5 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110

Serpin B5 (SERPINB5) Antibody
20-abx008867
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Cytochrome B5 (CYB5) Antibody
20-abx014018
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 Āµg
  • Shipped within 5-10 working days.

Ā 

Ā 

Improvement of a 3D thoughts extracellular matrix model to assessment the interaction between microglia and T cells in co-custom

 

Neurodegenerative points are characterised by the activation of brain-resident microglia cells and by the infiltration of peripheral T cells. Nonetheless, their interplay in sickness has not been clarified however. It is powerful to analysis sophisticated cell dynamics in dwelling animals, and straightforward two-dimensional (2D) cell custom fashions do not resemble the fragile 3D development of thoughts tissue. Subsequently, we developed a biomimetic 3D in vitro custom system for co-cultivation of microglia and T cells.

 

Given that activation and/or migration of immune cells inside the thoughts is probably affected by components of the extracellular matrix, outlined 3D fibrillar collagen I-based matrices have been constructed and modified with hyaluronan and/or chondroitin sulphate, resembling factors of thoughts extracellular matrix. Murine microglia and spleen-derived T cells have been cultured alone or in co-culture on the constructed matrices. Microglia exhibited in vivo-like morphology and T cells confirmed enhanced survival when co-cultured with microglia or to a minor diploma inside the presence of glia-conditioned medium.

 

The open and porous fibrillar development of the matrix allowed for cell invasion and direct cell-cell interaction, with stronger invasion of T cells. Every cell types confirmed no dependence on the matrix modifications. Microglia could be activated on the matrices by lipopolysaccharide resulting in interleukin-6 and tumour necrosis factor-Ī± secretion. The findings herein level out that biomimetic 3D matrices allow for co-cultivation and activation of main microglia and T cells and provide useful devices to assessment their interaction in vitro.

 

 

A two-dimensional multiwell cellculture method for the production of CYP3A4-expressing hepatocyte-like cells from HepaRG cells

 

Cytochrome P450 enzymes (CYP) function in drug metabolism inside the liver. To guage fairly a couple of drug candidates, a high-content screening (HCS) system with hepatocyte-like cells (HLCs) which will change grownup human hepatocytes is required. Human hepatocellular carcinoma HepaRG is the one cell line capable of providing HLCs with extreme CYP3A4 expression akin to that in grownup hepatocytes after cell differentiation.

 

The aim of this study was to design an ideal multiwell custom system for HLCs using transgenic HepaRG cells expressing the EGFP coding an enhanced inexperienced fluorescent protein beneath CYP3A4 transcriptional regulation. HLCs have been matured on 5 a number of varieties of 96-well black plates.

 

Culturing HLCs on glass-bottom Optical CVG plates significantly promoted cell maturation and elevated metabolic train by twofold beneath two-dimensional (2D) custom conditions, and these choices have been enhanced by 2% collagen coating.

 

Three plates for three-dimensional (3D) cell cultures with a gas-exchangeable material or dimethylpolysiloxane membrane bottom formed a lot of spherical colonies, whereas they’ve been ineffective for CYP3A4 expression. Under optimized conditions provided proper right here,

 

HLCs misplaced responsiveness to nuclear receptor-mediated transcriptional induction of CYP3A4, suggesting that CYP3A4 transcription has already been completely upregulated. Subsequently, HepaRG-derived HLCs will current another choice to human hepatocytes with extreme ranges of CYP3A4 enzyme train even beneath 2D custom conditions. This will improve numerous drug screening methods.

 

Gamborg's B-5 Medium; With Vitamins
CP011-500 50L
EUR 126
DC MEDIUM W/ BCIG
D04-116-10kg 10 kg
EUR 4539
DC MEDIUM W/ BCIG
D04-116-2Kg 2 Kg
EUR 1026
DC MEDIUM W/ BCIG
D04-116-500g 500 g
EUR 315
BME 100X Vitamins for Basal Medium Eagle (Modified)
BML01-100ML 100 ml
EUR 73
  • Product line: Animal Cell Culture Media
  • Product family: Basal Medium Eagle
Description: 100X Vitamins for Basal Medium Eagle (Modified)
BME 100X Vitamins for Basal Medium Eagle (Modified)
BML01-500ML 500 ml
EUR 92
  • Product line: Animal Cell Culture Media
  • Product family: Basal Medium Eagle
Description: 100X Vitamins for Basal Medium Eagle (Modified)
Gamborg's B-5 Medium; With Vitamins and Sucrose
CP012-010 10X1L
EUR 104
Gamborg's B-5 Medium; With Vitamins and Sucrose
CP012-500 50L
EUR 126
Schneider's Medium, w/ L-glutamine
CCM1318-500 500 mL
EUR 86.94
  • Product category: Culture Media
Medium 199, with L-Glutamine, w/ Earle's saltsĀ 
CCM2441-500 500 mL
EUR 68.07
  • Product category: Culture Media
Murashige and Skoog, With Vitamins
CP030-010 10X1L
EUR 99
Murashige and Skoog, With Vitamins
CP030-500 50L
EUR 126
Glutamate dehydrogenase (40 U/mg), Beef Liver, freeze dried powder
GDH-B5 20 mU
EUR 347
100 ML MEM VITAMINS 100X SOLUTION
25-020-CI 100 mL/pk
EUR 67
Description: Media Catalog; Cell Culture Reagents
Murashige and Skoog, With Gamborg's Vitamins
CP029-010 10X1L
EUR 113
Murashige and Skoog, With Gamborg's Vitamins
CP029-500 50L
EUR 138
Coxsackievirus (B5)
DAG4691 0.25 mg Ask for price
Procyanidin B5
TBW01326 unit Ask for price
Murashige and Skoog, With Vitamins and Glycine
CP031-010 10X1L
EUR 99
Murashige and Skoog, With Vitamins and Glycine
CP031-500 50L
EUR 126
Rat leukotriene B5(LT-B5) ELISA kit
CSB-EQ027966RA-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat leukotriene B5 (LT-B5) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat leukotriene B5(LT-B5) ELISA kit
1-CSB-EQ027966RA
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat leukotriene B5(LT-B5) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
BME 100X Amino Acids for Basal Medium Eagle (Modified). W/O L-glutamine.
BML02-500ML 500 ml
EUR 104
  • Product line: Animal Cell Culture Media
  • Product family: Basal Medium Eagle
Description: 100X Amino Acids for Basal Medium Eagle (Modified). Without L-glutamine.
Coxsackievirus B5 protein
30-1334 100 ug
EUR 398
Description: Purified native Coxsackievirus B5 protein (Faulkener Strain)
Cytochrome B5 antibody
20R-CG009 100 ul
EUR 457
Description: Goat polyclonal Cytochrome B5 antibody
Serpin B5 Antibody
34298-100ul 100ul
EUR 252
Serpin B5 Antibody
34298-50ul 50ul
EUR 187
Gluten Exorphin B5
5-01227 4 x 5mg Ask for price
Vitamin B5 [HRP]
DAGA-123H 1mg
EUR 1222
Vitamin B5 [KLH]
DAGA-123K 1mg
EUR 1235
Serpin B5 antibody
70R-50205 100 ul
EUR 244
Description: Purified Polyclonal Serpin B5 antibody
Serpin B5 antibody
70R-33858 100 ug
EUR 327
Description: Rabbit polyclonal Serpin B5 antibody
K6H6/B5 cells
C0033002 One Frozen vial
EUR 543
B5 Receptor Antibody
abx430923-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.
Cytochrome b5 Antibody
abx232186-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.
Gluten Exorphin B5
H-1666.0025 25.0mg
EUR 576
Description: Sum Formula: C30H38N6O7; CAS# [68382-18-3]
Gluten Exorphin B5
H-1666.0100 100.0mg
EUR 1663
Description: Sum Formula: C30H38N6O7; CAS# [68382-18-3]
anti-Cytochrome b5
YF-PA11223 50 ul
EUR 363
Description: Mouse polyclonal to Cytochrome b5
anti-Cytochrome b5
YF-PA23551 50 ul
EUR 334
Description: Mouse polyclonal to Cytochrome b5
Compound W
2208-250
EUR 300
Compound W
2208-50
EUR 115
W-G
5-02089 4 x 5mg Ask for price
W 54011
B6069-10 10 mg
EUR 393
Description: W 54011 is a potent and orally active non-peptide C5a receptor antagonist with Ki value of 2.2 nM [1].The complement C5a is a 74-amino acid peptide produced during complement activation processes.
W 54011
B6069-25 25 mg
EUR 857
Description: W 54011 is a potent and orally active non-peptide C5a receptor antagonist with Ki value of 2.2 nM [1].The complement C5a is a 74-amino acid peptide produced during complement activation processes.
W 54011
B6069-5 5 mg
EUR 232
Description: W 54011 is a potent and orally active non-peptide C5a receptor antagonist with Ki value of 2.2 nM [1].The complement C5a is a 74-amino acid peptide produced during complement activation processes.
Compound W
A4401-50 50 mg
EUR 177
Description: Inhibitor of ?-secretase; causes a decrease in the released levels of A?42 and notch-1 A?-like peptide 25 (N?25).
W-2429
HY-100174 1mg
EUR 567
W-54011
HY-16992A 10mM/1mL
EUR 288
Bcl-w
GT15196 100 ug
EUR 526
Opticlear W
NAT1484 EACH
EUR 158
Opticlear W
NAT1486 EACH
EUR 498
Mounting Medium
4300 3 ml
EUR 180.5
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.
VALI Medium
450 500 ml
EUR 265
BEGM Medium
451 500 ml
EUR 152
DC MEDIUM
D04-117-10kg 10 kg
EUR 1579
DC MEDIUM
D04-117-2kg 2kg
EUR 382
DC MEDIUM
D04-117-500g 500 g
EUR 140
EC MEDIUM
E05-100-10kg 10 kg
EUR 814
EC MEDIUM
E05-100-2Kg 2 Kg
EUR 216
EC MEDIUM
E05-100-500g 500 g
EUR 95
Advanced Medium
C0003-04 RT 500 mL Bottle
EUR 103
Medium 199
C0012-01 RT 500 mL Bottle
EUR 105
COLIFORM MEDIUM
C03-127-10kg 10 kg
EUR 1324
COLIFORM MEDIUM
C03-127-2kg 2kg
EUR 327
COLIFORM MEDIUM
C03-127-500g 500 g
EUR 125
Heller's Medium
CP014-010 10X1L
EUR 99
Heller's Medium
CP014-500 50L
EUR 126
Hoagland's Medium
CP015-010 10X1L
EUR 99
Hoagland's Medium
CP015-500 50L
EUR 126
HLP MEDIUM
H08-107-10kg 10 kg
EUR 2278
HLP MEDIUM
H08-107-2Kg 2 Kg
EUR 534
HLP MEDIUM
H08-107-500g 500 g
EUR 182
NeuroProgenitor Medium
NM42400 125 ml
EUR 304
SIM MEDIUM
S19-110-10kg 10 kg
EUR 1021
SIM MEDIUM
S19-110-2kg 2kg
EUR 261
SIM MEDIUM
S19-110-500g 500 g
EUR 107
SOB MEDIUM
S19-124-10kg 10 kg
EUR 965
SOB MEDIUM
S19-124-2kg 2kg
EUR 249
SOB MEDIUM
S19-124-500g 500 g
EUR 104
A Medium
DJ1018 100g
EUR 84.8
  • Product category: Culture Media/Medium
M9 Medium
SD7024 250g
EUR 71.75
  • Product category: Culture Media/Medium
M9CA Medium
SD7025 250g
EUR 71.75
  • Product category: Culture Media/Medium
YM Medium
SD7031 250g
EUR 70.45
  • Product category: Culture Media/Medium
TYGPN Medium
SD7032 250g
EUR 70.01
  • Product category: Culture Media/Medium
M63 Medium
SD7033 250g
EUR 71.75
  • Product category: Culture Media/Medium
Monoclonal Proinsulin Antibody (Clone HPI-B5), Clone: HPI-B5
AMR09558G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human Proinsulin (Clone HPI-B5). The antibodies are raised in Mouse and are from clone HPI-B5. This antibody is applicable in WB and IHC, E
Rat Neuropeptide W(NP-W)ELISA Kit
CSB-E17025r-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Neuropeptide W (NP-W) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat Neuropeptide W(NP-W)ELISA Kit
1-CSB-E17025r
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Rat Neuropeptide W(NP-W) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Phytic acid, 50% (w/w) in water
GK9311-100G 100 g
EUR 61
Phytic acid, 50% (w/w) in water
GK9311-25G 25 g
EUR 45
Beryllium fluoride, 33% (w/w) aqueous solution
GK1240-5G 5 g
EUR 190
Monoclonal GLP-1 Antibody (Clone HGL-B5), Clone: HGL-B5
AMM04792G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human GLP-1 (Clone HGL-B5). The antibodies are raised in Mouse and are from clone HGL-B5. This antibody is applicable in IF, E
Human Cytochrome b5 Antibody
33293-05111 150 ug
EUR 261
Polyclonal Serpin B5 Antibody
APR05559G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Serpin B5 . This antibody is tested and proven to work in the following applications:
Serpin B5 (SERPINB5) Antibody
20-abx008867
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Serpin B5 (SERPINB5) Antibody
20-abx115469
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.
Serpin B5 (SERPINB5) Antibody
20-abx127043
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.
Serpin B5 (SERPINB5) Antibody
abx038026-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.
Cytochrome B5 (CYB5) Antibody
20-abx014018
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 Āµg
  • Shipped within 5-10 working days.
Serpin B5 (SERPINB5) Antibody
20-abx014060
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 Āµg
  • Shipped within 5-10 working days.
Serpin B5 Blocking Peptide
20-abx063080
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
Gluten Exorphin B5 Peptide
20-abx265771
  • EUR 328.00
  • EUR 495.00
  • EUR 272.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.
Polyclonal anti-Cytochrome b5
Cb5 50 uL
EUR 280
Serpin B5 Conjugated Antibody
C34298 100ul
EUR 397
Serpin B5 (SERPINB5) Antibody
20-abx321461
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.
Serpin B5 (SERPINB5) Antibody
20-abx321596
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.
Homeobox B5 (HOXB5) Antibody
20-abx325423
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Cytochrome b5 Assay Kit
abx298800-100Assays 100 Assays
EUR 425
  • Shipped within 5-10 working days.
Cytochrome b5 Polyclonal Antibody
ABP51122-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of Cytochrome b5 from Human, Mouse, Rat. This Cytochrome b5 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
Cytochrome b5 Polyclonal Antibody
ABP51122-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of Cytochrome b5 from Human, Mouse, Rat. This Cytochrome b5 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
Cytochrome b5 Polyclonal Antibody
ABP51122-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of Cytochrome b5 from Human, Mouse, Rat. This Cytochrome b5 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Cytochrome b5 at AA range: 30-110
Serpin B5 (SERPINB5) Antibody
20-abx001093
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.
Cytochrome b5 Polyclonal Antibody
ES2121-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against Cytochrome b5 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Cytochrome b5 Polyclonal Antibody
ES2121-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against Cytochrome b5 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
anti- Cytochrome b5 antibody
FNab02186 100Āµg
EUR 505.25
  • Immunogen: cytochrome b5 type A(microsomal)
  • Uniprot ID: P00387
  • Research Area: Metabolism
Description: Antibody raised against Cytochrome b5
Anti-Cytochrome b5 antibody
PAab02186 100 ug
EUR 355
Human Vitamin B5(VB5)
QY-E05699 96T
EUR 361
Anti-Cytochrome b5 antibody
STJ92622 200 Āµl
EUR 197
Description: Rabbit polyclonal to Cytochrome b5.
Anti-Cytochrome b5 (4C2)
YF-MA10219 100 ug
EUR 363
Description: Mouse monoclonal to Cytochrome b5
Anti-Cytochrome b5 (1A8)
YF-MA10220 50 ug
EUR 363
Description: Mouse monoclonal to Cytochrome b5
Anti-ARHGEF5 (3A12-B5)
YF-MA16260 100 ug
EUR 363
Description: Mouse monoclonal to ARHGEF5
Inactivated Coxsackievirus B5 Antigen
VAng-Lsx0077-inquire inquire Ask for price
Description: Coxsackievirus (B5), natural protein.
Cathepsin W antibody
10R-6744 100 ug
EUR 705
Description: Mouse monoclonal Cathepsin W antibody
Cathepsin W antibody
10R-8036 100 ug
EUR 470
Description: Mouse monoclonal Cathepsin W antibody
W-13 hydrochloride
B6276-10 10 mg
EUR 216
W-13 hydrochloride
B6276-5 5 mg
EUR 158
W-5 hydrochloride
B6277-25 25 mg
EUR 234
W-5 hydrochloride
B6277-5 5 mg
EUR 116
W-7 hydrochloride
B6278-10 10 mg
EUR 112
W-7 hydrochloride
B6278-100 100 mg
EUR 206
W-7 hydrochloride
B6278-50 50 mg
EUR 144
W-9 hydrochloride
B6279-10 10 mg
EUR 276
W-9 hydrochloride
B6279-50 50 mg
EUR 999
W-84 dibromide
B6338-10 10 mg
EUR 284
W-84 dibromide
B6338-50 50 mg
EUR 1038
Cathepsin W Antibody
20-abx121048
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Cathepsin-W (Recombinant)
20-abx073419
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.
W-G Peptide
20-abx265714
  • EUR 328.00
  • EUR 495.00
  • EUR 272.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.
Bcl-W protein
E20-80116 20ug
EUR 408
W-5 hydrochloride
GL9804-25MG 25 mg
EUR 241
W-5 hydrochloride
GL9804-5MG 5 mg
EUR 126
anti-Cathepsin W
YF-PA23548 50 ul
EUR 334
Description: Mouse polyclonal to Cathepsin W
Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:
Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
ABP-MED-10004 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:
Mouse IgG1 Monoclonal Clone: HPI-B5 to Proinsulin (Clone HPI-B5)
3106-100 100 ug
EUR 355
  • What are the applications and the optimal dilutions of the product Antibody against PARP (Clone 10H)for analysis? - Immunocytochemistry : 5-20 ?g/ml | Western Blot (10 ?g/ml using colorimetric methods | <2 ?g/ml for ECL) | Immunoprecipitation and ELI
  • Show more
Description: This product represents Mouse IgG1 Monoclonal Clone: HPI-B5 against Proinsulin (Clone HPI-B5)for application in Indirect ELISA, Western blot, Immunohistochemistry.The immunogen is Recombinant full length insulin.This antibody can be used for detection of the target molecule in samples from Human, bovine, pig.
Murashige and Skoog, With Vitamins, Glycine, and 30g/L Sucrose
CP032-010 10X1L
EUR 101
Murashige and Skoog, With Vitamins, Glycine, and 30g/L Sucrose
CP032-500 50L
EUR 126
EverBrite Mounting Medium
23001 10mL
EUR 178
Description: Minimum order quantity: 1 unit of 10mL
Recovery Medium - 6x12mL
1711 4/PK
EUR 128
SuperMOUNT? Mounting Medium
1211-20
EUR 201
Aqueous Mounting Medium
AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 80

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