Presence of susceptible wild strains of Anopheles gambiae in a large industrial palm farm located in Aboisso, South-Eastern of Côte d’Ivoire.
The effectiveness of malaria management programmes by means of implementation of vector management actions is challenged by the emergence of insecticide resistance. In the South-Eastern area of Côte d’Ivoire, the place palm oil plantations stay the predominant agricultural crop, the susceptibility of wild Anopheles gambiae sensu lato species continues to be unknown and thus requires a explicit consideration.
The present examine was carried out to handle the hole by in-depth characterization of susceptibility degree of An. gambiae mosquitoes from Ehania-V1 to WHO-recommended doses of six pesticides belonging to accessible courses and in addition to display screen a subset for goal website mutations and doable inhibition of P450 enzymes.Overall outcomes confirmed variable resistance profile throughout WHO-recommended pesticides examined.
Mortalities ranged from 8.3% (the bottom mortality was recorded with DDT) to 98% (the best mortality was recorded with fenitrothion). Importantly, mortality to deltamethrin, an vital pyrethroid used in public well being for impregnation of mosquito nets was near 98%, precluding a doable susceptibility to this insecticide, albeit additional investigations are required.
Pre-exposure of An. gambiae s.l. to PBO didn’t present any vital variation throughout pesticides (p = 0.002), though a partial improve was detected for alphacypermethrin and bendiocarb, suggesting a low of exercise of cytochrome P450 enzymes (p = 0.277). High frequency of kdr L1014F was recorded in each Anopheles coluzzii (91%) and in An. gambiae (96%), related to ace-1 R G119S mutation at low frequency (<20%).
The excessive mortality price to deltamethrin, organophosphate and the non-detection of P450 exercise in resistance noticed in Ehania-V1 seems as a constructive final result for additional management methods as metabolic-based P450 resistance stays main problem to handle. These outcomes ought to assist the National Malaria Control Programme when designing methods for vector management in palm oil areas of Côte d’Ivoire.